| First Author | Harada M | Year | 2001 |
| Journal | Immunology | Volume | 104 |
| Issue | 1 | Pages | 67-74 |
| PubMed ID | 11576222 | Mgi Jnum | J:71602 |
| Mgi Id | MGI:2150475 | Doi | 10.1046/j.0019-2805.2001.01278.x |
| Citation | Harada M, et al. (2001) Evidence of the extrathymic development of tyrosinase-related protein-2-recognizing CD8+ T cells with low avidity. Immunology 104(1):67-74 |
| abstractText | The majority of the human tumour-associated antigens characterized to date are derived from non-mutated self-proteins. However, nothing is known about the development of autoreactive and tumour-associated antigen-recognizing T cells. Tyrosinase-related protein (TRP)-2 is a non-mutated melanocyte differentiation antigen and TRP-2-recognizing CD8+ T cells are known to show responses to melanoma both in humans and mice. In addition, TRP-2-reactive T cells with low avidity have been suggested to be readily induced from the spleen cells of naive mice. On the other hand, recent reports suggest that self antigen-reactive CD8+ T cells can be positively selected in the periphery. In this study, we tested the possibility that TRP-2-reactive CD8+ T cells in naive mice could develop via the extrathymic pathway. As a consequence, TRP-2-reactive CD8+ T cell precursors in naive C57BL/6 mice were suggested to express both interleukin-2 (IL-2) receptor beta chain (IL-2Rbeta) and CD44 molecules, in a manner similar to that of extrathymically developed T cells. Furthermore, IL-2Rbeta+ CD44+ CD8+ T cells were detected in the adult thymectomized and bone marrow-reconstituted mice, and functional TRP-2-reactive T cells were generated from their spleen cells. Overall, these results suggest that low avidity CD8+ T cells recognizing TRP-2 can be developed extrathymically. |
