Primary Identifier | MGI:6157024 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Cabp2 |
Transmission | Germline | Strain of Origin | C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct, containing the tdTomato red fluorescent marker gene and a neomycin resistance gene cassette, was inserted immediately downstream of the ATG translation start site in exon 1. The construct replaces the coding region of exon 1 and exon 2a. Stop codons were introduced in all three reading frames of exon 2b. RT-PCR and immunohistochemistry experiments confirmed the lack of transcript and protein expression in the retina from this allele. |