| First Author | Hayakawa M | Year | 2021 |
| Journal | Sci Rep | Volume | 11 |
| Issue | 1 | Pages | 14824 |
| PubMed ID | 34290295 | Mgi Jnum | J:313828 |
| Mgi Id | MGI:6740061 | Doi | 10.1038/s41598-021-94307-0 |
| Citation | Hayakawa M, et al. (2021) Characterization and visualization of murine coagulation factor VIII-producing cells in vivo. Sci Rep 11(1):14824 |
| abstractText | Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which enhanced green fluorescent protein (EGFP) was expressed instead of the F8 gene. EGFP-positive FVIII-producing cells existed only in thin sinusoidal layer of the liver and characterized as CD31(high), CD146(high), and lymphatic vascular endothelial hyaluronan receptor 1 (Lyve1)(+). EGFP-positive cells can be clearly distinguished from lymphatic endothelial cells in the expression profile of the podoplanin(-) and C-type lectin-like receptor-2 (CLEC-2)(+). In embryogenesis, EGFP-positive cells began to emerge at E14.5 and subsequently increased according to liver maturation. Furthermore, plasma FVIII could be abolished by crossing F8 conditional deficient mice with Lyve1-Cre mice. In conclusion, in mice, FVIII is only produced from endothelial cells exhibiting CD31(high), CD146(high), Lyve1(+), CLEC-2(+), and podoplanin(-) in liver sinusoidal endothelial cells. |
