First Author | Thomas KA | Year | 1981 |
Journal | J Biol Chem | Volume | 256 |
Issue | 17 | Pages | 9156-66 |
PubMed ID | 7263706 | Mgi Jnum | J:32363 |
Mgi Id | MGI:79862 | Doi | 10.1016/s0021-9258(19)52522-4 |
Citation | Thomas KA, et al. (1981) The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor. J Biol Chem 256(17):9156-66 |
abstractText | The complete amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor has been determined from analyses of the peptides generated by cyanogen bromide, trypsin, and chymotrypsin from the naturally occurring fragments. All peptides were sequenced automatically in a spinning-cup sequenator using Polybrene to minimize extractive losses by the solvents employed thoughout the degradation cycles. The gamma-subunit, a serine protease with arginine specificity, contains 233 amino acid residues and shares sequence homology with other proteases of this family. The five disulfide bonds of the gamma-subunit are a subset of the six disulfides present in bovine trypsin, as judged by the location of the half-cystine residues in the primary structure. An N-linked carbohydrate side chain is attached to Asn-78 in at least a majority of tee gamma-molecules. |