Primary Identifier | MGI:3706369 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | P4ha1 |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 2 is replaced by a lacZ/neo cassette. RT-PCR analysis confirmed the absence of mRNA in homozygous embryos at E10.5. Enzymatic activity, as determined in an activity assay with radioactively-labeled substrate, was reduced to 20% of normal activity. |