| First Author | Fukuda T | Year | 2006 |
| Journal | Genesis | Volume | 44 |
| Issue | 4 | Pages | 159-67 |
| PubMed ID | 16604518 | Mgi Jnum | J:109498 |
| Mgi Id | MGI:3629023 | Doi | 10.1002/dvg.20201 |
| Citation | Fukuda T, et al. (2006) Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2. Genesis 44(4):159-67 |
| abstractText | BMP signaling plays pleiotropic roles in various tissues. Transgenic mouse lines that overexpress BMP signaling in a tissue-specific manner would be beneficial; however, production of each tissue-specific transgenic mouse line is labor-intensive. Here, using a Cre-loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre-expression mouse lines, Cre-mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue-specific manner without having to generate independent tissue-specific transgenic lines. |
