| First Author | Lee A | Year | 2000 |
| Journal | Genomics | Volume | 70 |
| Issue | 3 | Pages | 354-63 |
| PubMed ID | 11161786 | Mgi Jnum | J:66993 |
| Mgi Id | MGI:1929581 | Doi | 10.1006/geno.2000.6404 |
| Citation | Lee A, et al. (2000) The Human Renal Sodium Sulfate Cotransporter (SLC13A1; hNaSi-1) cDNA and Gene: Organization, Chromosomal Localization, and Functional Characterization. Genomics 70(3):354-63 |
| abstractText | Sulfate plays an essential role during growth, development, bone/cartilage formation, and cellular metabolism. In this study, we have determined the structure of the human Na(+)-sulfate cotransporter (hNaSi-1) cDNA (Human Genome Nomenclature Committee-approved symbol SLC13A1) and gene (NAS1). hNaSi-1 encodes a protein of 595 amino acids with 13 putative transmembrane domains. hNaSi-1 mRNA expression was exclusive to the human kidney. Expression of hNaSi-1 protein in Xenopus oocytes demonstrated a high-affinity Na(+)-sulfate cotransporter that was inhibited by selenate, thiosulfate, molybdate, tungstate, citrate, and succinate. Antisense inhibition experiments suggest hNaSi-1 to represent the major Na(+)-sulfate cotransporter in the human kidney. NAS1 was localized on human chromosome 7, mapped to 7q31-q32, near the sulfate transporter genes, DRA and SUT-1. The NAS1 gene contains 15 exons, spanning over 83 kb in length. Knowledge of the structure, function, and chromosomal localization of hNaSi-1 will permit the screening of NAS1 mutations in humans with disorders in renal sulfate reabsorption and homeostasis. Copyright 2000 Academic Press. |
