| Primary Identifier | MGI:6454709 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Ptges |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using a BAC from the RPCI-23 library for the targeting vector, the endogenous genomic sequence, starting from the translation initiation site in exon 1 and ending with exon 2, was replaced with a human PTGES mini-gene consisting of the full-length cDNA with human intron 1 inserted between the exon 1 and 2 cDNA sequences. An F3 site flanked puromycin resistance gene cassette was inserted downstream of the mini-gene. This puro cassette was later removed through flp-mediated recombination. |
