| First Author | Wang T | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 498 |
| Issue | 1 | Pages | 139-145 |
| PubMed ID | 29421662 | Mgi Jnum | J:271453 |
| Mgi Id | MGI:6280170 | Doi | 10.1016/j.bbrc.2018.02.027 |
| Citation | Wang T, et al. (2018) Deficiency of alpha Calcitonin-gene-related peptide impairs peri-implant angiogenesis and osseointegration via suppressive vasodilative activity. Biochem Biophys Res Commun 498(1):139-145 |
| abstractText | Blood supply is essential for the bone healing process to obtain successful osseointegration. alpha Calcitonin gene-related peptide (alphaCGRP) is osteoanabolic and is the most potent microvascular vasodilator currently known with validated angiogenic effect in vitro. We previously demonstrated that lentiviral alphaCGRP overexpression vector system could express the gene effectively to enhance titanium implant osseointegration. In this study, we investigated the effect of alphaCGRP on peri-implant angiogenesis during healing process in vivo. Based on investigation of blood vessel alteration in the peri-implant region of wild-type mice, we found a reduced angiogenesis alongside a decline in bone-implant contact percentage and bone mass in alphaCGRP (-/-) mice. Overexpression of alphaCGRP could partly rescue the impairment. Alterations were also detected in three-dimensional vascular structural parameters and expression of bone and vessel related genes. The results showed alphaCGRP increased vascular volume fraction and mean vessel size, as well as spatially relocated vessels approximate to the region of bone formation. And angiogenic and osteogenic genes were significantly upregulated in the transfection and alphaCGRP(+/+) group. These results suggested that alphaCGRP played a synergic role in angiogenesis and osseointegration, partly as a consequence of its vasodilative activity. |
