| First Author | Wang B | Year | 2019 |
| Journal | Cell Transplant | Volume | 28 |
| Issue | 11 | Pages | 1420-1431 |
| PubMed ID | 31426665 | Mgi Jnum | J:347428 |
| Mgi Id | MGI:6459402 | Doi | 10.1177/0963689719871000 |
| Citation | Wang B, et al. (2019) alphaCGRP Affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway. Cell Transplant 28(11):1420-1431 |
| abstractText | Alpha-calcitonin gene-related peptide (alphaCGRP) plays a significant pathophysiological role in the regulation of bone metabolism. Our previous research indicated that alphaCGRP might have a potential application in enhancing osseointegration in vivo. To further uncover the intrinsic mechanism of its networks in bone regeneration, here we investigate the impact of alphaCGRP on osteogenic differentiation in bone marrow-derived mesenchymal stem cells (BMSCs) from both wild-type and alphaCGRP(-/-) mice. Considering the half-life of alphaCGRP in plasma is only 10 min, we applied alphaCGRP lentivirus and stably transfected it into BMSCs, followed by transfection identification and cell cycle assay. We further conducted a series of in vitro tests, and the results revealed that biological functions including migratory ability and osteogenicity exhibited positive correlation with BMSCs' alphaCGRP expression. Meanwhile, this phenomenon was associated with an enhanced expression of YAP (Yes-associated protein), the key downstream effector of the Hippo pathway. To sum up, our data together with previous in vivo observations is likely to elucidate the intrinsic mechanism of alphaCGRP in bone remodeling, and alphaCGRP would appear to be a novel treatment to promote bone wound healing. |
