Primary Identifier | MGI:3612442 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Abcd2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 4 through 6 were deleted. Western blot showed a complete lack of protein in all tissues of mutant mice. Quantitative RT-PCR confirmed absence of full-length transcript, although shorter, smir-type and low expressed fragments were detected, consistent with nonsense-mediated mRNA decay process. |