First Author | Matsushima Y | Year | 1999 |
Journal | Digestion | Volume | 60 |
Issue | 1 | Pages | 34-40 |
PubMed ID | 9892796 | Mgi Jnum | J:53005 |
Mgi Id | MGI:1331177 | Doi | 10.1159/000007586 |
Citation | Matsushima Y, et al. (1999) Augmentation of water-immersion stress- induced gastric mucosal lesions in BALB/c mice infected with Helicobacter felis. Digestion 60(1):34-40 |
abstractText | BACKGROUND AND AIMS: Inoculation of Helicobacter felis into the murine stomach has been reported to induce chronic gastric inflammation and may be a model of Helicobacter pylori-induced chronic gastritis. In this study, to characterize H. felis-induced gastritis, the gastric production of interleukin-1beta (IL-1beta) and hepatocyte growth factor (HGF) was measured in mice. METHODS: Gastric mucosal lesions were induced in H. felis-infected BALB/c mice by water-immersion stress. The severity score of gastric erosions per stomach was measured as the sum of the length of erosions. Gene expression of IL-1beta and HGF were analyzed by Northern blot analysis and production of HGF was examined using the enzyme immunoassay method. RESULTS: Water-immersion stress induced gastric mucosal lesions accompanied by increased expression of IL-1beta mRNA. H. felis infection evoked enhanced expression of IL-1beta and HGF genes. When H. felis-infected mice were stressed by water immersion, the mucosal lesions were more severe than those in non-infected mice. Moreover, IL-1beta gene expression as well as HGF production was further increased. CONCLUSIONS: Although H. felis inoculation did not cause gastric mucosal erosions by itself, it augmented the stress-induced erosions. |