| First Author | Stein P | Year | 2000 |
| Journal | Mol Reprod Dev | Volume | 55 |
| Issue | 3 | Pages | 241-8 |
| PubMed ID | 10657042 | Mgi Jnum | J:60312 |
| Mgi Id | MGI:1353155 | Doi | 10.1002/(SICI)1098-2795(200003)55:3<241::AID-MRD1>3.0.CO;2-T |
| Citation | Stein P, et al. (2000) Initiation of a chromatin-based transcriptionally repressive state in the preimplantation mouse embryo: lack of a primary role for expression of somatic histone H1. Mol Reprod Dev 55(3):241-8 |
| abstractText | A chromatin-based transcriptionally repressive state develops during the two-cell stage in preimplantation mouse embryos. Correlated with the initial formation of this state is the expression of somatic histone H1, which could confer repression by promoting the formation of a transcriptionally repressive chromatin structure. To ascertain if the expression of histone H1 could play such a primary role in initiating the formation of this transcriptionally repressive state, the endogenous pool of somatic histone H1 in the two-cell embryo was greatly expanded by injection of 25 or 100 pg of histone H1 at the one-cell stage. The expression of the transcription-requiring complex, which is an accepted marker for genome activation, was then assessed during the two-cell stage. No significant inhibition was noted following the injection of 25 pg of histone H1. A transient inhibition was observed following injection of 100 pg, but this was likely due to a delay in cleavage to the two-cell stage. We conclude that it is unlikely that the expression of somatic histone H1 is a major factor in the initial establishment of the chromatin-based transcriptionally repressive state that accompanies genome activation. Copyright 2000 Wiley-Liss, Inc. |
