| First Author | Mauduit O | Year | 2022 |
| Journal | Cell Rep | Volume | 39 |
| Issue | 2 | Pages | 110663 |
| PubMed ID | 35417692 | Mgi Jnum | J:355254 |
| Mgi Id | MGI:7283923 | Doi | 10.1016/j.celrep.2022.110663 |
| Citation | Mauduit O, et al. (2022) A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep 39(2):110663 |
| abstractText | Fibroblast growth factor 10 (FGF10) is well established as a mesenchyme-derived growth factor and a critical regulator of fetal organ development in mice and humans. Using a single-cell RNA sequencing (RNA-seq) atlas of salivary gland (SG) and a tamoxifen inducible Fgf10(CreERT2):R26-tdTomato mouse, we show that FGF10(pos) cells are exclusively mesenchymal until postnatal day 5 (P5) but, after P7, there is a switch in expression and only epithelial FGF10(pos) cells are observed after P15. Further RNA-seq analysis of sorted mesenchymal and epithelial FGF10(pos) cells shows that the epithelial FGF10(pos) population express the hallmarks of ancient ionocyte signature Forkhead box i1 and 2 (Foxi1, Foxi2), Achaete-scute homolog 3 (Ascl3), and the cystic fibrosis transmembrane conductance regulator (Cftr). We propose that epithelial FGF10(pos) cells are specialized SG ionocytes located in ducts and important for the ionic modification of saliva. In addition, they maintain FGF10-dependent gland homeostasis via communication with FGFR2b(pos) ductal and myoepithelial cells. |
