|  Help  |  About  |  Contact Us

Allele : Prl8a2<em1(icre)Fjd> prolactin family 8, subfamily a, member 2; endonuclease-mediated mutation 1, Franco J DeMayo
Primary Identifier  MGI:7311604 Allele Type  Endonuclease-mediated
Attribute String  Recombinase Gene  Prl8a2
Transmission  Germline Strain of Origin  (129S6/SvEvTac x C57BL/6NCrl)F1
Is Recombinase  true Is Wild Type  false
molecularNote  Using CRISPR/Cas9 endonuclease-mediated genome editing, a donor plasmid designed to insert a codon improved cre recombinase (iCre) and a polyadenylation sequence immediately downstream of the start codon of the prolactin family 8, subfamily a, member 2 (Prl8a2) gene, a single guide RNA (ACTAGCAGTCTTATCCAACCNGG), and the cas9 nuclease with a puromycin resistance sequence were transfected into G4 embryonic stem (ES) cells.
  • mutations:
  • Insertion
  • synonyms:
  • Prl8a2iCre,
  • Prl8a2iCre
Paste the following link
Quick Links:
SummaryGenomePhenotypeOther
 
Quick Links:
SummaryGenomePhenotypeOther
 
Lists
This Allele isn't in any lists. Upload a list.

1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

1 Driven By

Trail: Allele

4 Publication categories

Trail: Allele




MouseMine is a collaboration between MGI at The Jackson Laboratory and the InterMine project at the Cambridge Systems Biology Centre. MouseMine is funded through a grant from the NIH/NHGRI.The Jackson Laboratory makes no representation about the suitability or accuracy of this software or data for any purpose, and makes no warranties, either express or implied, including merchantability and fitness for a particular purpose or that the use of this software or data will not infringe any third party patents, copyrights, trademarks, or other rights. the software and data are provided "as is". This software and data are provided to enhance knowledge and encourage progress in the scientific community and are to be used only for research and educational purposes. Any reproduction or use for commercial purpose is prohibited without the prior express written permission of the Jackson Laboratory.

Copyright © 2017 by The Jackson Laboratory. All Rights Reserved

© 2002 - 2017 Department of Genetics, University of Cambridge, Downing Street,
Cambridge CB2 3EH, United Kingdom