| First Author | Wilder PJ | Year | 2001 |
| Journal | Gene | Volume | 270 |
| Issue | 1-2 | Pages | 201-9 |
| PubMed ID | 11404017 | Mgi Jnum | J:70142 |
| Mgi Id | MGI:2136500 | Doi | 10.1016/s0378-1119(01)00475-9 |
| Citation | Wilder PJ, et al. (2001) Isolation and characterization of the murine transforming growth factor-beta2 promoter. Gene 270(1-2):201-9 |
| abstractText | This report describes the isolation and characterization of the 5' flanking region of the murine transforming growth factor beta-2 (TGF-beta2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage P1 genomic library. The resulting clone was sequenced and compared to promoters for the human and chicken TGF-beta2 genes. The sequence located near the transcription start site is highly conserved. It includes a TATA box, an E-box, and a largely conserved CRE/ATF site. A series of murine TGF-beta2 promoter/reporter constructs was generated to identify regulatory regions of the gene. As in the case of the human TGF-beta2 gene, sequences just upstream of the TATA box, including the CRE/ATF site, actively stimulate the murine TGF-beta2 promoter. However, unlike the human TGF-beta2 gene, the 5' flanking region of the murine TGF-beta2 gene contains a long alternating purine/pyrimidine repeat that unexpectedly exerts a strong positive effect on its promoter. This is of particular interest since alternating purine/pyrimidine repeats in other promoters have been observed to be inhibitory. |
