First Author | Yamashita A | Year | 2000 |
Journal | FEBS Lett | Volume | 481 |
Issue | 2 | Pages | 147-51 |
PubMed ID | 10996314 | Mgi Jnum | J:64789 |
Mgi Id | MGI:1889983 | Doi | 10.1016/s0014-5793(00)01987-6 |
Citation | Yamashita A, et al. (2000) Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis. FEBS Lett 481(2):147-51 |
abstractText | SoxLZ/Sox6, a member of the Sox protein family, contains a leucine zipper motif in addition to an HMG box, which is its DNA binding domain. Here we have identified a novel SoxLZ/Sox6 binding protein, termed Solt, which we obtained independently using both a far-Western blot and a yeast two-hybrid screen. Like SoxLZ/Sox6 mRNA, Solt mRNA was exclusively expressed in the testis in mouse. Solt contains an unusual leucine zipper, which bound to the leucine zipper region of SoxLZ/Sox6 in vitro. In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca(2+)/calmodulin-dependent protein kinase IV. |