Primary Identifier | MGI:2177435 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | E2f3 |
Transmission | Germline | Strain of Origin | 129S2/SvPas |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A PGK-neomycin resistance cassette replaced sequences encoding amino acids 134-294, which comprise the nuclear localization signal, cyclin A binding, DNA binding, and the leucine zipper domains, and introduced an in-frame termination codon just upstream of the cassette. Western blot analysis did not detect protein in MEF cells derived from homozygous mutant embryos. |