| First Author | Gu X | Year | 2018 |
| Journal | Cell Rep | Volume | 23 |
| Issue | 13 | Pages | 3878-3890 |
| PubMed ID | 29949771 | Mgi Jnum | J:270753 |
| Mgi Id | MGI:6278687 | Doi | 10.1016/j.celrep.2018.05.091 |
| Citation | Gu X, et al. (2018) Synchronized Astrocytic Ca(2+) Responses in Neurovascular Coupling during Somatosensory Stimulation and for the Resting State. Cell Rep 23(13):3878-3890 |
| abstractText | The role of astrocytes in neurovascular coupling (NVC) is unclear. Here, we applied a multimodality imaging approach to concomitantly measure synchronized neuronal or astrocytic Ca(2+) and hemodynamic changes in the mouse somatosensory cortex at rest and during sensory electrical stimulation. Strikingly, we found that low-frequency stimulation (0.3-1 Hz), which consistently evokes fast neuronal Ca(2+) transients (6.0 +/- 2.7 ms latency) that always precede vascular responses, does not always elicit astrocytic Ca(2+) transients (313 +/- 65 ms latency). However, the magnitude of the hemodynamic response is increased when astrocytic transients occur, suggesting a facilitatory role of astrocytes in NVC. High-frequency stimulation (5-10 Hz) consistently evokes a large, delayed astrocytic Ca(2+) accumulation (3.48 +/- 0.09 s latency) that is temporarily associated with vasoconstriction, suggesting a role for astrocytes in resetting NVC. At rest, neuronal, but not astrocytic, Ca(2+) fluctuations correlate with hemodynamic low-frequency oscillations. Taken together, these results support a role for astrocytes in modulating, but not triggering, NVC. |
