| First Author | Geng Y | Year | 2007 |
| Journal | Mol Cell | Volume | 25 |
| Issue | 1 | Pages | 127-39 |
| PubMed ID | 17218276 | Mgi Jnum | J:119079 |
| Mgi Id | MGI:3701151 | Doi | 10.1016/j.molcel.2006.11.029 |
| Citation | Geng Y, et al. (2007) Kinase-independent function of cyclin E. Mol Cell 25(1):127-39 |
| abstractText | E-type cyclins are thought to drive cell-cycle progression by activating cyclin-dependent kinases, primarily CDK2. We previously found that cyclin E-null cells failed to incorporate MCM helicase into DNA prereplication complex during G(0) --> S phase progression. We now report that a kinase-deficient cyclin E mutant can partially restore MCM loading and S phase entry in cyclin E-null cells. We found that cyclin E is loaded onto chromatin during G(0) --> S progression. In the absence of cyclin E, CDT1 is normally loaded onto chromatin, whereas MCM is not, indicating that cyclin E acts between CDT1 and MCM loading. We observed a physical association of cyclin E with CDT1 and with MCMs. We propose that cyclin E facilitates MCM loading in a kinase-independent fashion, through physical interaction with CDT1 and MCM. Our work indicates that-in addition to their function as CDK activators-E cyclins play kinase-independent functions in cell-cycle progression. |
