| Primary Identifier | MGI:7531287 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Nsf |
| Transmission | Germline | Strain of Origin | C57BL/6NSlc |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector PG00174_Z_5_D06 obtained from KOMP inserted between exons 5 and 6 and is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6-7 at position 103914303. The critical exons 6-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. |
