| First Author | Olivier M | Year | 2014 |
| Journal | Biochim Biophys Acta | Volume | 1841 |
| Issue | 12 | Pages | 1741-51 |
| PubMed ID | 25462452 | Mgi Jnum | J:230451 |
| Mgi Id | MGI:5760097 | Doi | 10.1016/j.bbalip.2014.10.003 |
| Citation | Olivier M, et al. (2014) ABCG1 is involved in vitamin E efflux. Biochim Biophys Acta 1841(12):1741-51 |
| abstractText | Vitamin E membrane transport has been shown to involve the cholesterol transporters SR-BI, ABCA1 and NPC1L1. Our aim was to investigate the possible participation of another cholesterol transporter in cellular vitamin E efflux: ABCG1. In Abcgl-deficient mice, vitamin E concentration was reduced in plasma lipoproteins whereas most tissues displayed a higher vitamin E content compared to wild-type mice. alpha- and gamma-tocopherol efflux was increased in CHO cells overexpressing human ABCG1 compared to control cells. Conversely, alpha- and gamma- tocopherol efflux was decreased in ABCG1-knockdown human cells (Hep3B hepatocytes and THP-1 macro- phages). Interestingly, alpha- and gamma-tocopherol significantly downregulated ABCG1 and ABCA1 expression levels in Hep3B and THP-1, an effect confirmed in vivo in rats given vitamin E for 5 days. This was likely due to reduced LXR activation by oxysterols, as Hep3B cells and rat liver treated with vitamin E displayed a significantly reduced content in oxysterols compared to their respective controls. Overall, the present study reveals for the first time that ABCG1 is involved in cellular vitamin E efflux. |
