Primary Identifier | MGI:7628164 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Mpc1 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | LoxP sites were inserted around exons 3-5 and an IRES-nls-eYFP, lox511 flanked stop signal, luciferase gene and FRT flanked selection cassette were inserted downstream of exon 5 via homologous recombination. Flp mediated recombination removed the selection cassette. Cre mediated recombination reomved exons 3-5 and the lox511 flanked stop signal creating a null allele and allowing expression of luciferase. |