| First Author | Ninomiya K | Year | 2007 |
| Journal | Biochem Biophys Res Commun | Volume | 362 |
| Issue | 2 | Pages | 460-6 |
| PubMed ID | 17714690 | Mgi Jnum | J:125173 |
| Mgi Id | MGI:3757799 | Doi | 10.1016/j.bbrc.2007.07.193 |
| Citation | Ninomiya K, et al. (2007) Osteoclastic activity induces osteomodulin expression in osteoblasts. Biochem Biophys Res Commun 362(2):460-6 |
| abstractText | Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity. |
