| First Author | Yan H | Year | 2019 |
| Journal | Mol Med Rep | Volume | 19 |
| Issue | 5 | Pages | 4468-4474 |
| PubMed ID | 30942442 | Mgi Jnum | J:290311 |
| Mgi Id | MGI:6442054 | Doi | 10.3892/mmr.2019.10095 |
| Citation | Yan H, et al. (2019) Long noncoding RNA NEAT1 sponges miR125a5p to suppress cardiomyocyte apoptosis via BCL2L12. Mol Med Rep 19(5):4468-4474 |
| abstractText | Increasing evidence has suggested that long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) has critical roles in multiple biological processes; however, few studies have reported on its function in heart disease. The present study indicated that NEAT1 expression is markedly downregulated in cardiomyocytes following ischemia/reperfusion injury in vivo and hydrogen peroxide treatment in vitro. Further experiments suggested that ectopic overexpression of NEAT1 suppresses cardiomyocyte apoptosis induced by hydrogen peroxide, as assessed by TUNEL assay and flow cytometry. In addition, using a dualluciferase reporter assay, NEAT1 was demonstrated to directly interact with microRNA (miR)125a5p and overexpression of miR125a5p efficiently reversed the stimulatory effect of NEAT1 on Bcell lymphoma2like 12 (BCL2L12) expression. Furthermore, the results indicated that NEAT1 inhibits cardiomyocyte apoptosis via regulating the expression of BCL2L12, which appeared to be mediated via miR125a5p. In conclusion, the present study suggested that NEAT1 functions as a miR sponge to inhibit cardiomyocyte apoptosis and may be a novel therapeutic target for cardiomyocyte apoptosisassociated heart diseases. |
