First Author | Wardell BB | Year | 1993 |
Journal | Mamm Genome | Volume | 4 |
Issue | 2 | Pages | 109-12 |
PubMed ID | 8431634 | Mgi Jnum | J:3885 |
Mgi Id | MGI:52390 | Doi | 10.1007/BF00290435 |
Citation | Wardell BB, et al. (1993) The identification of Y chromosome-linked markers with random sequence oligonucleotide primers. Mamm Genome 4(2):109-12 |
abstractText | The polymerase chain reaction (PCR)-based technique of random amplification of polymorphic DNA (RAPD) is extremely useful for developing DNA-based markers. We previously identified a linkage group of eight unmapped RAPD markers that distinguish C57BL/6J and DBA/2J mice (Mammalian Genome 3: Woodward et al., 73-78, 1992). In this study, we report that all eight markers are Y Chromosome (Chr)-linked. One additional Y-linked RAPD was discovered serendipitously during the screening of a C3H/HeJ x (C3H/HeJ x SJL/J)F1 BC1 population. The segregation of all nine markers was analyzed with a panel of 14 independent inbred strains of male mice. The nine markers could be divided into three distinct groups: (1) DYByu2, DYByu5, DYByu6, and DYByu8 identify both the M.m. musculus and M.m. domesticus type Y Chr; (2) DYByu1, DYByu3, DYByu4, and DYByu7 are specific for the M.m. musculus type; and (3) DYByu9 is specific for the M.m. domesticus type. The results clearly indicate that the RAPD technique can be used to identify Y Chr-linked, DNA-based markers in mammalian species. |