| First Author | Moxham CM | Year | 1993 |
| Journal | Science | Volume | 260 |
| Issue | 5110 | Pages | 991-5 |
| PubMed ID | 8493537 | Mgi Jnum | J:127313 |
| Mgi Id | MGI:3763562 | Doi | 10.1126/science.8493537 |
| Citation | Moxham CM, et al. (1993) Induction of G alpha i2-specific antisense RNA in vivo inhibits neonatal growth. Science 260(5110):991-5 |
| abstractText | Guanosine triphosphate-binding regulatory proteins (G proteins) are key elements in transmembrane signaling and have been implicated as regulators of more complex biological processes such as differentiation and development. The G protein G alpha i2 is capable of mediating the inhibitory control of adenylylcyclase and regulates stem cell differentiation to primitive endoderm. Here an antisense RNA to G alpha i2 was expressed in a hybrid RNA construct whose expression was both tissue-specific and induced at birth. Transgenic mice in which the antisense construct was expressed displayed a lack of normal development in targeted organs that correlated with the absence of G alpha i2. The loss of G alpha i2 expression in adipose tissue of the transgenic mice was correlated with a rise in basal levels of adenosine 3',5'-monophosphate (cAMP) and the loss of receptor-mediated inhibition of adenylylcyclase. These data expand our understanding of G protein function in vivo and demonstrate the necessity for G alpha i2 in the development of liver and fat. |
