| First Author | Wang Y | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 449 |
| Issue | 2 | Pages | 229-34 |
| PubMed ID | 24832732 | Mgi Jnum | J:222005 |
| Mgi Id | MGI:5643842 | Doi | 10.1016/j.bbrc.2014.05.012 |
| Citation | Wang Y, et al. (2014) 24S,25-Epoxycholesterol in mouse and rat brain. Biochem Biophys Res Commun 449(2):229-34 |
| abstractText | 24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4mug/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20mug/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7alpha-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide. |
