| First Author | Harwig SS | Year | 1995 |
| Journal | J Clin Invest | Volume | 95 |
| Issue | 2 | Pages | 603-10 |
| PubMed ID | 7860744 | Mgi Jnum | J:23715 |
| Mgi Id | MGI:71406 | Doi | 10.1172/JCI117704 |
| Citation | Harwig SS, et al. (1995) Bactericidal properties of murine intestinal phospholipase A2. J Clin Invest 95(2):603-10 |
| abstractText | We purified a molecule from the murine small intestine that killed both Escherichia coli and Listeria monocytogenes, and identified it as intestinal phospholipase A2 (iPLA2) by NH2-terminal sequencing and enzymatic measurements. The ability of iPLA2 to kill. L. monocytogenes was greatly enhanced by 5 mM calcium, inhibited by EGTA and abolished after reduction and alkylation, suggesting that enzymatic activity was required for iPLA2-mediated bactericidal activity. A mouse-avirulent phoP mutant, S. typhimurium 7953S, was 3.5-fold more susceptible to iPLA2 than its isogenic virulent parent, S. typhimurium 14028S (estimated minimal bactericidal concentrations 12.7 +/- 0.5 micrograms/ml vs. 43.9 +/- 4.5 micrograms/ml P < 0.001). Overall, these findings identify iPLA2 as part of the antimicrobial arsenal that equips Paneth cells to protect the small intestinal crypts from microbial invasion. Because iPLA2 is identical to Type 2 phospholipase A2 molecules found in other sites, including spleen, platelets and inflammatory exudate cells, this enzyme may also contribute to antibacterial defenses elsewhere in the body. |
