| First Author | Schneider A | Year | 2004 |
| Journal | Genesis | Volume | 40 |
| Issue | 1 | Pages | 7-14 |
| PubMed ID | 15354288 | Mgi Jnum | J:92301 |
| Mgi Id | MGI:3052368 | Doi | 10.1002/gene.20048 |
| Citation | Schneider A, et al. (2004) Generation of a conditional allele of the mouse prostaglandin EP(4) receptor. Genesis 40(1):7-14 |
| abstractText | Genetic disruption of the mouse EP(4) receptor results in perinatal lethality associated with persistent patent ductus areteriosus (PDA). To circumvent this, an EP(4) allele amenable to conditional deletion using the Cre/loxP system was generated. The targeting construct was comprised of a floxed exon2 in tandem with the neomycin-resistance gene in intron 2, flanked by third 3' LoxP site. Mice homozygous for the targeted allele (EP(4) (lox+neo/lox+neo)), or following its Cre-mediated deletion (EP(4) (del/del)), also die within hours of birth with PDA. In contrast, mice homozygous for a partially recombined allele, retaining exon2 but lacking neo (EP(4) (flox/flox)), are viable and show no overt phenotype. Postnatal deletion of the floxed EP(4) gene is efficiently achieved in the liver and kidney in a transgenic mouse expressing the inducible Mx1Cre recombinase. The EP(flox) (4) mouse should provide a useful reagent with which to examine the physiologic roles of the EP(4) receptor. genesis 40:7-14, 2004. Copyright 2004 Wiley-Liss, Inc. |
