| First Author | He Y | Year | 2013 |
| Journal | J Immunol | Volume | 190 |
| Issue | 1 | Pages | 334-9 |
| PubMed ID | 23225887 | Mgi Jnum | J:190815 |
| Mgi Id | MGI:5449757 | Doi | 10.4049/jimmunol.1202737 |
| Citation | He Y, et al. (2013) TLR Agonists Stimulate Nlrp3-Dependent IL-1beta Production Independently of the Purinergic P2X7 Receptor in Dendritic Cells and In Vivo. J Immunol 190(1):334-9 |
| abstractText | On the basis of studies in mouse macrophages, activation of the nucleotide-binding oligomerization domain-like receptor (NLR) pyrin domain-containing 3 (Nlrp3) inflammasome is thought to require two signals. The first signal is provided by TLR stimulation and triggers the synthesis of the IL-1beta precursor and Nlrp3. The second signal can be mediated by stimulation of the purinergic receptor P2X ligand-gated ion channel 7 (P2X7) by millimolar concentrations of ATP. However, these high concentrations of ATP are not found normally in the in vivo extracellular milieu, raising concern about the physiological relevance of the ATP-P2X7 pathway of inflammasome activation. In this article, we show that unlike macrophages, murine bone marrow-derived and splenic dendritic cells (DCs) can secrete substantial amounts of mature IL-1beta upon stimulation with TLR ligands in the absence of ATP stimulation. The differential ability of DCs to release IL-1beta and activate caspase-1 was associated with increased expression of Nlrp3 under steady-state conditions and of pro-IL-1beta and Nlrp3 after stimulation with TLR agonists. IL-1beta secretion from stimulated DCs was largely dependent on the Nlrp3 inflammasome, but independent of P2X7 and unaffected by incubation with apyrase. More importantly, i.p. administration of LPS induced IL-1beta production in serum, which was abrogated in Nlrp3-null mice but was unaffected in P2X7-deficient mice. These results demonstrate differential regulation of the Nlrp3 inflammasome in macrophages and DCs. Furthermore, they challenge the idea that the ATP-P2X7 axis is critical for TLR-induced IL-1beta production via the Nlrp3 inflammasome in vivo. |
