| First Author | Menezes S | Year | 2016 |
| Journal | Immunity | Volume | 45 |
| Issue | 6 | Pages | 1205-1218 |
| PubMed ID | 28002729 | Mgi Jnum | J:288060 |
| Mgi Id | MGI:6141554 | Doi | 10.1016/j.immuni.2016.12.001 |
| Citation | Menezes S, et al. (2016) The Heterogeneity of Ly6C(hi) Monocytes Controls Their Differentiation into iNOS(+) Macrophages or Monocyte-Derived Dendritic Cells. Immunity 45(6):1205-1218 |
| abstractText | Inflammation triggers the differentiation of Ly6C(hi) monocytes into microbicidal macrophages or monocyte-derived dendritic cells (moDCs). Yet, it is unclear whether environmental inflammatory cues control the polarization of monocytes toward each of these fates or whether specialized monocyte progenitor subsets exist before inflammation. Here, we have shown that naive monocytes are phenotypically heterogeneous and contain an NR4A1- and Flt3L-independent, CCR2-dependent, Flt3(+)CD11c(-)MHCII(+)PU.1(hi) subset. This subset acted as a precursor for FcgammaRIII(+)PD-L2(+)CD209a(+), GM-CSF-dependent moDCs but was distal from the DC lineage, as shown by fate-mapping experiments using Zbtb46. By contrast, Flt3(-)CD11c(-)MHCII(-)PU.1(lo) monocytes differentiated into FcgammaRIII(+)PD-L2(-)CD209a(-)iNOS(+) macrophages upon microbial stimulation. Importantly, Sfpi1 haploinsufficiency genetically distinguished the precursor activities of monocytes toward moDCs or microbicidal macrophages. Indeed, Sfpi1(+/-) mice had reduced Flt3(+)CD11c(-)MHCII(+) monocytes and GM-CSF-dependent FcgammaRIII(+)PD-L2(+)CD209a(+) moDCs but generated iNOS(+) macrophages more efficiently. Therefore, intercellular disparities of PU.1 expression within naive monocytes segregate progenitor activity for inflammatory iNOS(+) macrophages or moDCs. |
