| First Author | Chen ZC | Year | 2000 |
| Journal | Mol Immunol | Volume | 37 |
| Issue | 8 | Pages | 455-66 |
| PubMed ID | 11090880 | Mgi Jnum | J:66155 |
| Mgi Id | MGI:1928039 | Doi | 10.1016/s0161-5890(00)00064-x |
| Citation | Chen ZC, et al. (2000) Genes coding evolutionary novel anti-carbohydrate antibodies: studies on anti-Gal production in alpha1,3galactosyltransferase knock out mice. Mol Immunol 37(8):455-66 |
| abstractText | This study analyzes the gene repertoire coding for antibodies to an evolutionary novel immunogenic carbohydrate antigen in mice. The alpha-gal epitope (Galalpha1-3Galbeta1-4GlcNAc-R) is an autoantigen, abundantly expressed in wild type mice, but absent in alpha1, 3galactosyltransferase knock-out (KO) mice, where it can induce the production of the anti-Gal antibody. Hybridoma clones secreting anti-Gal were isolated from different mice and their immunoglobulin genes were analyzed. All anti-Gal clones were found to be encoded by the heavy chain gene VH22.1 and light chain gene VK5.1. Moreover, one 'forbidden' anti-Gal clone, produced in a wild type mouse, was also encoded by VH 22.1 and VK 5.1. The genes coding for the different anti-Gal clones were found to contain somatic mutations and different CDR3 domains. These data imply that a highly restricted gene usage combined with junctional diversity and somatic mutations can generate new antibodies that have not been produced in the course of the evolution of a species. |
