| First Author | Greer YE | Year | 2014 |
| Journal | Mol Biol Cell | Volume | 25 |
| Issue | 10 | Pages | 1629-40 |
| PubMed ID | 24648492 | Mgi Jnum | J:220879 |
| Mgi Id | MGI:5636600 | Doi | 10.1091/mbc.E13-10-0598 |
| Citation | Greer YE, et al. (2014) Casein kinase 1delta functions at the centrosome and Golgi to promote ciliogenesis. Mol Biol Cell 25(10):1629-40 |
| abstractText | Inhibition of casein kinase 1 delta (CK1delta) blocks primary ciliogenesis in human telomerase reverse transcriptase immortalized retinal pigmented epithelial and mouse inner medullary collecting duct cells-3. Mouse embryonic fibroblasts (MEFs) and retinal cells from Csnk1d (CK1delta)-null mice also exhibit ciliogenesis defects. CK1delta catalytic activity and centrosomal localization signal (CLS) are required to rescue cilia formation in MEFs(Csnk1d null). Furthermore, expression of a truncated derivative containing the CLS displaces full-length CK1delta from the centrosome and decreases ciliary length in control MEFs, suggesting that centrosomal CK1delta has a role in ciliogenesis. CK1delta inhibition also alters pericentrosomal or ciliary distribution of several proteins involved in ciliary transport, including Ras-like in rat brain-11A, Ras-like in rat brain-8A, centrosomal protein of 290 kDa, pericentriolar material protein 1, and polycystin-2, as well as the Golgi distribution of its binding partner, A-kinase anchor protein 450 (AKAP450). As reported for AKAP450, CK1delta was required for microtubule nucleation at the Golgi and maintenance of Golgi integrity. Overexpression of an AKAP450 fragment containing the CK1delta-binding site inhibits Golgi-derived microtubule nucleation, Golgi distribution of intraflagellar transport protein 20 homologue, and ciliogenesis. Our results suggest that CK1delta mediates primary ciliogenesis by multiple mechanisms, one involving its centrosomal function and another dependent on its interaction with AKAP450 at the Golgi, where it is important for maintaining Golgi organization and polarized trafficking of multiple factors that mediate ciliary transport. |
