| First Author | Takeda Y | Year | 2017 |
| Journal | Int J Mol Sci | Volume | 18 |
| Issue | 6 | PubMed ID | 28561797 |
| Mgi Jnum | J:271745 | Mgi Id | MGI:6282136 |
| Doi | 10.3390/ijms18061162 | Citation | Takeda Y, et al. (2017) TRAV7-2*02 Expressing CD8(+) T Cells Are Responsible for Palladium Allergy. Int J Mol Sci 18(6):1162 |
| abstractText | While metallic biomaterials have led to an improvement in the quality of life, metal allergies, especially to palladium (Pd), has caused a recent increase in allergic patients. Metal allergy is known to be a T cell-mediated delayed-type hypersensitivity (DTH); however, the pathogenic T cell subsets and the specific T cell receptor (TCR) have not been identified. Therefore, we attempted to identify the pathogenic T cells responsible for Pd allergy. We found that activating CD8(+) T cells significantly increased and that the TRAV (TCRalpha variable) 7-2*02 chain skewed in Pd allergic mice. Furthermore, adoptive transfer experiments revealed that in vitro-cultured Pd-stimulated antigen presenting cells (APCs) function as memory APCs with recipient mice developing Pd allergy and that the frequency of TRAV7-2*02 increases the same as conventional Pd allergic mice. In contrast, neither proliferation of CD8(+) T cells nor increasing of TRAV7-2*02 was observed in major histocompatibility complex I (MHC I)-deficient Pd-APCs transferred to mice. Taken together, we revealed that TRAV7-2*02-expressing CD8(+) T cells are the pathogenic T cells for the development of Pd allergy. We also identified the CDR3 consensus motif of pathogenic TCRs as CAAXSGSWQLIF in TRAV7-2*02/TRAJ (TCRalpha junction)22*01 positive cells. These results suggest that the specific TCRs represent novel targets for the development of diagnostics and treatments for metal allergy. |
