| First Author | Pham TP | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 413 |
| Issue | 2 | Pages | 376-82 |
| PubMed ID | 21893041 | Mgi Jnum | J:177524 |
| Mgi Id | MGI:5295341 | Doi | 10.1016/j.bbrc.2011.08.110 |
| Citation | Pham TP, et al. (2011) Berberine exerts anti-adipogenic activity through up-regulation of C/EBP inhibitors, CHOP and DEC2. Biochem Biophys Res Commun 413(2):376-82 |
| abstractText | Berberine exerts an anti-adipogenic activity that is associated with the down-regulation of C/EBPalpha and PPARgamma. Stimulation of AMP-activated kinase (AMPK) caused by inhibition of mitochondrial respiration has been suggested to underlie such molecular regulation. In the present study, we show that berberine up-regulated the expression of two different sets of C/EBP inhibitors, CHOP and DEC2, while down-modulating C/EBPalpha, PPARgamma and other adipogenic markers and effectors in differentiating 3T3-L1 preadipocytes and mature adipocytes. Data also suggested that the berberine-induced up-regulation of CHOP and DEC2 was attributable to selective activation of an unfolded protein response (UPR) and modified extracellular environment, respectively. As a result, the anti-adipogenic activity of berberine was diminished remarkably by adjusting the differentiation culture media and limitedly but consistently by knockdown of CHOP expression. Together, up-regulation of C/EBP inhibitors appears to underlie the berberine-induced repression of C/EBPalpha and PPARgamma and, so, the inhibition of adipogenesis. |
