| First Author | Lange SS | Year | 2012 |
| Journal | Nucleic Acids Res | Volume | 40 |
| Issue | 10 | Pages | 4473-82 |
| PubMed ID | 22319213 | Mgi Jnum | J:197718 |
| Mgi Id | MGI:5494372 | Doi | 10.1093/nar/gks054 |
| Citation | Lange SS, et al. (2012) DNA polymerase zeta is required for proliferation of normal mammalian cells. Nucleic Acids Res 40(10):4473-82 |
| abstractText | Unique among translesion synthesis (TLS) DNA polymerases, pol zeta is essential during embryogenesis. To determine whether pol zeta is necessary for proliferation of normal cells, primary mouse fibroblasts were established in which Rev3L could be conditionally inactivated by Cre recombinase. Cells were grown in 2% O(2) to prevent oxidative stress-induced senescence. Cells rapidly became senescent or apoptotic and ceased growth within 3-4 population doublings. Within one population doubling following Rev3L deletion, DNA double-strand breaks and chromatid aberrations were found in 30-50% of cells. These breaks were replication dependent, and found in G1 and G2 phase cells. Double-strand breaks were reduced when cells were treated with the reactive oxygen species scavenger N-acetyl-cysteine, but this did not rescue the cell proliferation defect, indicating that several classes of endogenously formed DNA lesions require Rev3L for tolerance or repair. T-antigen immortalization of cells allowed cell growth. In summary, even in the absence of external challenges to DNA, pol zeta is essential for preventing replication-dependent DNA breaks in every division of normal mammalian cells. Loss of pol zeta in slowly proliferating mouse cells in vivo may allow accumulation of chromosomal aberrations that could lead to tumorigenesis. Pol zeta is unique amongst TLS polymerases for its essential role in cell proliferation. |
