| First Author | Erickson RP | Year | 1983 |
| Journal | Genetics | Volume | 105 |
| Issue | 1 | Pages | 181-9 |
| PubMed ID | 6578171 | Mgi Jnum | J:7189 |
| Mgi Id | MGI:55660 | Doi | 10.1093/genetics/105.1.181 |
| Citation | Erickson RP, et al. (1983) Identification of an autosomal locus affecting steroid sulfatase activity among inbred strains of mice. Genetics 105(1):181-9 |
| abstractText | We have found an activity variant for testicular and liver steroid sulfatase among inbred strains of mice that is not X-linked. C57BL/6J, SM/J and SWR/J testicular extracts hydrolyze 3H-dehydroepiandrosterone sulfate twice as rapidly as do A/J extracts. The C3H/HeJ and DBA/2J strains were intermediate. The Km values for C57BL/6J and A/J are 2.29 +/- 0.10 and 1.01 +/- 0.02 microM, respectively. The F1 values in both directions were intermediate, which argues against X-linkage of this trait. F2 values show scattered high-intermediate-low values compatible with assay variation superimposed on the segregation of codominant alleles. When assayed for both testicular and liver steroid sulfatase, nine recombinant inbred lines between A/J and C57BL/6J segregate to near the parental strain values. Thus, this activity variation for steroid sulfatase appears to be determined by a single gene, which is not X-linked. Sex and steroidal hormone differences in liver steroid sulfatase activity were not present in the A/J strain, but females of the C57BL/6J and some recombinant inbred lines had higher levels. Electrophoretic studies only disclosed a variant in the SM/J strain, which seems to be secondary to the well-known neuraminidase variation in SM/J. |
