| First Author | Iraha S | Year | 2018 |
| Journal | Stem Cell Reports | Volume | 10 |
| Issue | 3 | Pages | 1059-1074 |
| PubMed ID | 29503091 | Mgi Jnum | J:280609 |
| Mgi Id | MGI:6367889 | Doi | 10.1016/j.stemcr.2018.01.032 |
| Citation | Iraha S, et al. (2018) Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation. Stem Cell Reports 10(3):1059-1074 |
| abstractText | Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets. |
