Primary Identifier | MGI:2451310 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Wasf1 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = JSI-1. |
molecularNote | Exon 4 was replaced with a cassette containing both uracil- and neomycin-resistance genes. The insertion deleted 95 bp, including the splice donor site of exon 4, and introduced a nonsense mutation at codon 175. Normal protein was undetected by Western blot analysis of homozygous mutant brain tissue. |