First Author | Kumagai T | Year | 2009 |
Journal | Biochem Biophys Res Commun | Volume | 379 |
Issue | 2 | Pages | 456-9 |
PubMed ID | 19114028 | Mgi Jnum | J:144516 |
Mgi Id | MGI:3831059 | Doi | 10.1016/j.bbrc.2008.12.078 |
Citation | Kumagai T, et al. (2009) Early lethality of beta-1,4-galactosyltransferase V-mutant mice by growth retardation. Biochem Biophys Res Commun 379(2):456-9 |
abstractText | The beta-1,4-galactosyltransferase (beta-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, beta-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5(-/-) mice die by E10.5 while B4galt5(+/-) mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5(-/-) embryos but their appearance at E10.5 is close to that of B4galt5(+/-) embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5(-/-) embryos when compared to B4galt5(+/-) embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects. |