| First Author | Osterwalder M | Year | 2010 |
| Journal | Nat Methods | Volume | 7 |
| Issue | 11 | Pages | 893-5 |
| PubMed ID | 20953177 | Mgi Jnum | J:201720 |
| Mgi Id | MGI:5515355 | Doi | 10.1038/nmeth.1521 |
| Citation | Osterwalder M, et al. (2010) Dual RMCE for efficient re-engineering of mouse mutant alleles. Nat Methods 7(11):893-5 |
| abstractText | We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner. |
