| Primary Identifier | MGI:5085552 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Dnajc30 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-Regeneron |
| description | Cell lines 16795A-A9 and 16795A-F1 were successfully used to make chimeric mice. Germline transmission was accomplished. J:266469 Two independent lines of knockout mice were created from ES cell clones 16795A-F1 (line 1) and 16795A-A9 (line 2). Both lines exhibited similar patterns of expression and gross phenotypes. J:266469 focused primarily on line 2 generated from clone 16795A-A9. |
| molecularNote | The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 749bp between positions 135093108-135093856 of Chromosome 5 (Build GRCm39). Droplet digital PCR (ddPCR) analysis confirmed that Dnajc30 expression was severely reduced in homozygous mutant mice. |
