| First Author | Stein O | Year | 2002 |
| Journal | Biochem Biophys Res Commun | Volume | 290 |
| Issue | 5 | Pages | 1376-81 |
| PubMed ID | 11820773 | Mgi Jnum | J:74685 |
| Mgi Id | MGI:2158969 | Doi | 10.1006/bbrc.2002.6358 |
| Citation | Stein O, et al. (2002) Macrophage cholesterol efflux to free apoprotein A-I in C3H and C57BL/6 mice. Biochem Biophys Res Commun 290(5):1376-81 |
| abstractText | Cholesterol efflux from peritoneal macrophages of mice C57BL/6 susceptible and C3H resistant to atherosclerosis was compared, using apoprotein A-I as acceptor. The elicited macrophages were labeled with 3H-cholesterol and cholesterol enriched by incubation for 24 h with acetylated LDL. After incubation for 6 or 24 h, 3H-cholesterol efflux to free apoA-I (10 microg/ml) was significantly higher with macrophages derived from C3H mice compared to C57BL/6 mice. The cells were also pretreated with 0.3-0.45 mM cyclic AMP, 10 microM 9-cis-retinoic acid or 10 microM 22(R)-hydroxycholesterol, RXR and LXR ligands. Treatment with cyclic AMP, RXR, or LXR ligands, resulted in enhancement of 3H-cholesterol efflux in both strains. Under all conditions, 3H-cholesterol efflux was significantly higher in C3H compared to C57BL/6 macrophages. In conclusion, the higher cholesterol efflux from C3H macrophages could contribute toward the resistance of this strain to diet-induced atherosclerosis despite hypercholesterolemia. |
