Primary Identifier | MGI:5903087 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Cic |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
Project Collection | KOMP-CSD |
molecularNote | The L1L2_Bact_P cassette was inserted at position 25287449 of Chromosome 7. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site inserted into intron 8. A third loxP site is inserted in intron 11. Cre-mediated recombination removed the loxP-flanked portion of the construct, leaving a 5'FRT site, the En2 splice acceptor-IRES-lacZ-pA cassette, and a 3' loxP site. |