| First Author | Ohmura-Hoshino M | Year | 2009 |
| Journal | J Immunol | Volume | 183 |
| Issue | 11 | Pages | 6893-7 |
| PubMed ID | 19917682 | Mgi Jnum | J:157516 |
| Mgi Id | MGI:4430994 | Doi | 10.4049/jimmunol.0902178 |
| Citation | Ohmura-Hoshino M, et al. (2009) Cutting edge: requirement of MARCH-I-mediated MHC II ubiquitination for the maintenance of conventional dendritic cells. J Immunol 183(11):6893-7 |
| abstractText | MARCH-I (membrane-associated RING-CH I) has been suggested as a physiological E3 ubiquitin ligase for both MHC class II (MHC II) and B7-2. In this study, we show that MARCH-I-mediated MHC II ubiquitination is necessary for the maintenance of conventional dendritic cell (cDC) functions in the steady state. MARCH-I-deficient cDCs accumulated MHC II and B7-2 and exhibited low Ag-presenting ability for exogenous Ags and low cytokine-producing ability upon stimulation in vivo. Importantly, MHC II, but not B7-2, was required for impaired cDC function induced by loss of MARCH-I in vivo. Moreover, MHC II knockin mice whose MHC II was not ubiquitinated showed dysfunction of cDC similar to that of MARCH-I knockout mice. These results suggest that the accumulation of MHC II resulting from loss of ubiquitination caused cDC abnormality; therefore, MARCH-I may function as a housekeeper of cDC in the steady state. |
