| First Author | Li RC | Year | 2018 |
| Journal | Proc Natl Acad Sci U S A | Volume | 115 |
| Issue | 21 | Pages | 5570-5575 |
| PubMed ID | 29735665 | Mgi Jnum | J:262494 |
| Mgi Id | MGI:6159181 | Doi | 10.1073/pnas.1803443115 |
| Citation | Li RC, et al. (2018) Ca(2+)-activated Cl current predominates in threshold response of mouse olfactory receptor neurons. Proc Natl Acad Sci U S A 115(21):5570-5575 |
| abstractText | In mammalian olfactory transduction, odorants activate a cAMP-mediated signaling pathway that leads to the opening of cyclic nucleotide-gated (CNG), nonselective cation channels and depolarization. The Ca(2+) influx through open CNG channels triggers an inward current through Ca(2+)-activated Cl channels (ANO2), which is expected to produce signal amplification. However, a study on an Ano2(-/-) mouse line reported no elevation in the behavioral threshold of odorant detection compared with wild type (WT). Subsequent studies by others on the same Ano2(-/-) line, nonetheless, found subtle defects in olfactory behavior and some abnormal axonal projections from the olfactory receptor neurons (ORNs) to the olfactory bulb. As such, the question regarding signal amplification by the Cl current in WT mouse remains unsettled. Recently, with suction-pipette recording, we have successfully separated in frog ORNs the CNG and Cl currents during olfactory transduction and found the Cl current to predominate in the response down to the threshold of action-potential signaling to the brain. For better comparison with the mouse data by others, we have now carried out similar current-separation experiments on mouse ORNs. We found that the Cl current clearly also predominated in the mouse olfactory response at signaling threshold, accounting for approximately 80% of the response. In the absence of the Cl current, we expect the threshold stimulus to increase by approximately sevenfold. |
