Primary Identifier | MGI:5905012 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Sik2 |
Transmission | Germline | Strain of Origin | 129S2/SvPas |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An frt site flanked hygromycin resistance gene cassette was inserted in intron 4. Exon 5 was flanked by loxP sites. Exon 5 codes for part of the active catalytic domain including the Thr175 phosphorylation site within the activation loop. The hygro cassette was removed through subsequent FLP-mediated recombination, leaving a conditional-ready allele with a floxed exon 5. |