| First Author | Sasaki M | Year | 1996 |
| Journal | Endocr J | Volume | 43 |
| Issue | 1 | Pages | 45-52 |
| PubMed ID | 8732451 | Mgi Jnum | J:32763 |
| Mgi Id | MGI:80253 | Doi | 10.1507/endocrj.43.45 |
| Citation | Sasaki M, et al. (1996) Prolactin-dependent growth and gamma-casein gene expression in Ba/F3 cells transfected with a long form of mouse mammary prolactin receptor. Endocr J 43(1):45-52 |
| abstractText | Complementary DNA (cDNA) encoding a long form of prolactin receptor (PRL-RL) was cloned from mouse mammary gland by PCR using primers designed from the noncoding regions of previously reported rat ovarian PRL-RL cDNA. The nucleotide sequence encoding the extracellular and transmembrane domains of PRL-RL is completely identical to that of short forms of mouse PRL-R. The amino acid sequence deduced from the nucleotide sequence of mouse PRL-RL is 91% identical to that of rat PRL-RL. To address the question of whether or not the cloned mouse PRL-RL cDNA encodes a functional PRL-RL we transfected Ba/F3 IL-3-dependent murine pro-B lymphoid cells with the cDNA. By culturing the transfected cells in a medium which contained PRL in place of IL-3, we selected 5 PRL-dependent clones. All of these PRL-dependent clones, BaF/PD cells, expressed PRL-RL mRNA. In addition, BaF/PD cells expressed mammary-specific gamma-casein mRNA in response to PRL and dexamethasone. Based on these results, it was concluded that the mouse mammary PRL-RL cDNA cloned in this study is functionally active in mediating both PRL-dependent growth and mammary-specific gene expression. |
