| First Author | Kim JY | Year | 2022 |
| Journal | Cell Rep | Volume | 39 |
| Issue | 7 | Pages | 110821 |
| PubMed ID | 35584664 | Mgi Jnum | J:325214 |
| Mgi Id | MGI:7284012 | Doi | 10.1016/j.celrep.2022.110821 |
| Citation | Kim JY, et al. (2022) Twist2-driven chromatin remodeling governs the postnatal maturation of dermal fibroblasts. Cell Rep 39(7):110821 |
| abstractText | Dermal fibroblasts lose stem cell potency after birth, which prevents regenerative healing. However, the underlying intracellular mechanisms are largely unknown. We uncover the postnatal maturation of papillary fibroblasts (PFs) driven by the extensive Twist2-mediated remodeling of chromatin accessibility. A loss of the regenerative ability of postnatal PFs occurs with decreased H3K27ac levels. Single-cell transcriptomics, assay for transposase-accessible chromatin sequencing (ATAC-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) reveal the postnatal maturation trajectory associated with the loss of the regenerative trajectory in PFs, which is characterized by a marked decrease in chromatin accessibility and H3K27ac modifications. Histone deacetylase inhibition delays spontaneous chromatin remodeling, thus maintaining the regenerative ability of postnatal PFs. Genomic analysis identifies Twist2 as a major regulator within chromatin regions with decreased accessibility during the postnatal period. When Twist2 is genetically deleted in dermal fibroblasts, the intracellular cascade of postnatal maturation is significantly delayed. Our findings reveal the comprehensive intracellular mechanisms underlying intrinsic postnatal changes in dermal fibroblasts. |
